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Cultivation of Microorganisms: Types of Culture Medium

Solid, liquid, gel (semisolid) designed to support the growth and supply nutrients to any cell or microorganism is a culture medium. It is a special medium used in microbiological laboratories to grow different microorganisms. A microbiological culture medium must contain: Source of energy (chemicals for chemotrophs and lights for phototrophs) …

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General Methods of Microbial Isolation

In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, in order to identify the microbes of interest. Isolation can be done from, for example, water or soil flora or from living beings with skin …

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Measurement of Microbial Growth

Direct microscopic count The direct total cell count involves counting the number of cells in a simple microscopically. This technique is very similar to that used in blood cell counts. Scientists perform a direct microscopic count using a specially designed slide called a Petroff-Hausser cell counter/ counting chamber/ cytometer/ hemacytometer …

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