Generation Time The time required for a cell to divide or a population to divide is known as the generation time. For bacteria it is the time, required for a complete fission cycle. Generation time depends on nutrient media, pH, temperature etc. The generation time “g” can be determined from …

Solid, liquid, gel (semisolid) designed to support the growth and supply nutrients to any cell or microorganism is a culture medium. It is a special medium used in microbiological laboratories to grow different microorganisms. A microbiological culture medium must contain: Source of energy (chemicals for chemotrophs and lights for phototrophs) …

In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, in order to identify the microbes of interest. Isolation can be done from, for example, water or soil flora or from living beings with skin …