fbpx

Lactophenol Cotton Blue: Most Widely Used Stain in Mycology

Introduction

Lactophenol cotton blue (LPCB) is a widely used staining agent used in laboratories to observe chitin walled organisms (e.g. Fungi). It generally gives the specimen a blue color under microscope and helps to find them easily. It is also very simple to prepare.

Composition

This mounting medium is composed of three chemicals.

  • Lactophenol which is a mounting liquid. It carries both lactic acid (1) and phenol (2).
  • Cotton Blue (3) which helps to dye the specimen.
  1. Lactic acid preserves the fungal structure and clears the tissue.

2. Phenol, present in the first chemical kills the organisms (disinfectant behavior) and also prevent the cells from lysis. It usually deactivates the cellular lytic enzymes present in the specimen fungi.

3. Cotton blue targets the chitin present in the cell wall of the fungi and thus stain them (mainly the spores and hyphae).

Aspergillus sp. observed under Lactophenol Cotton Blue stain

Source:

  1. http://himedialabs.com/td/s016.pdf
Print Friendly, PDF & Email

About Abulais Shomrat

Abulais Shomrat
Currently in 4th year (Hons) in the Department of Botany, University of Dhaka. Planning to have multiple careers one by one but promised to be with 'Plantlet' as long as it's primary stage remains unfinished.

Check Also

Importance of Fungi part II

Beneficial role of fungi 5. Fungi in Agriculture: Beneficial aspects iii. Fungi as a biofertilizer …

Subscribe
Notify of
guest
0 Comments
Inline Feedbacks
View all comments
0
Would love your thoughts, please comment.x
()
x