Pathogenicity Test: Diagnosis of an Unknown Fungal Disease

For diagnosis of plant disease, it is essential to determine whether the disease is caused by a pathogen or an environmental factor i.e. abiotic factor. It is also important to know whether the disease is infectional or non-infectional.

Diagnosis of a disease caused by either a virus, viroids, rickettsia or protozoa may involve a series of tests. The most common of which are –

    Get Free Netflix Now

    Best safe and secure cloud storage with password protection

    GPL Themes For Free

    Get Envato Elements, Prime Video, Hotstar and Netflix For Free

    Best Money Earning Website 100$ Day

    Best ever Chat Forum

    #1 Top ranking article submission website

  1. Inoculation of several host plants with the pathogen and comparison of the symptoms on these plant/hosts with those produced on the same host by known pathogen.
  2. Electron microscopy of infected tissues & comparison of the morphology of the pathogen in them – if found with that of other known pathogens.
  3. Application of certain antibiotics on the diseased plant to determine whether the pathogen is susceptible to any of them or not as expressed by the recovery of the plant from the disease.
    For example – Susceptibility to tetracyclin would tend to indicate a possible MLO etiology. Susceptibility to penicillin (from fungus) would indicate a possible Rickettsia like bacter etiology, while no effect would indicate possible viral etiology.
  4. Thermotherapy of the disease by exposed diseased plants or parts of them to hot water in high temperatures for different periods of time. Recovery of symptoms at lower temperature  or in shorter periods would tend to indicate MLO or Rickettsia like etiology.
  5. Serology: If the pathogens can be isolated and purified e.g. Virus, Spiroplasma citrr cultures, antiserum may be produced & diagnostic subsequently used for diagnostic serological tests.

Revised by 

  1. Md. Siddiq Hasan on 25 August, 2020
Print Friendly, PDF & Email
0 0 votes
Article Rating

About Plantlet

This is an official account of this website. This ID holds articles from two categories. 1. Articles from our non-authors. 2. Articles of the authors whose IDs were deleted.

Check Also

Fungi: Sexual Reproduction

Fungi are microscopic/macroscopic eukaryotic and heterotrophic organisms exhibiting growth on various natural and synthetic substrates …

Notify of
Inline Feedbacks
View all comments
Would love your thoughts, please comment.x