What is Micro-propagation? Micropropagation is a method to produce genetically identical plantlets by using in vitro tissue culture techniques. More specifically, Micropropagation refers to the in vitro multiplication and/or regeneration of plant material under aseptic and controlled environmental conditions to produce thousands or millions of plants for transfer to the …

Metabolism: (Gr. metabole = change) the totality of the chemical changes in living cells which involves the buildup and breakdown of chemical compounds. Metabolites Primary metabolites: Molecules that are essential for growth and development of an organism. Secondary metabolites: Molecules that are not essential for growth and development of an …

Algal toxins are toxic substances released by some types of algae when they are present in large quantities (Blooms) and decay or degrade. These toxins are called ‘Phycotoxins’ (from Greek, phykos, “seaweed”; and toxikon, “toxin”). They are usually complex allelopathic metabolites produced by algal secondary metabolic pathways. These metabolites are (in most cases) not harmful to …

The primary aim of culture preservation is to maintain the organism alive, uncontaminated, and without variation or mutation, that is, to preserve the culture in a condition that is as close as possible to the original isolate. Once a microorganism has been isolated and grown in pure culture, it becomes …

Control of Microorganism by Heat Heat generally appears to kill micro-organisms by denaturing their enzymes: the resultant changes to the three-dimensional shapes of these proteins inactivate them. Heat resistance varies among different microbes. These differences can be expressed through the followings; Thermal death point (TDP) is the lowest temperature at …

Generation Time The time required for a cell to divide or a population to divide is known as the generation time. For bacteria it is the time, required for a complete fission cycle. Generation time depends on nutrient media, pH, temperature etc. The generation time “g” can be determined from …

Solid, liquid, gel (semisolid) designed to support the growth and supply nutrients to any cell or microorganism is a culture medium. It is a special medium used in microbiological laboratories to grow different microorganisms. A microbiological culture medium must contain: Source of energy (chemicals for chemotrophs and lights for phototrophs) …

In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, in order to identify the microbes of interest. Isolation can be done from, for example, water or soil flora or from living beings with skin …

Direct microscopic count The direct total cell count involves counting the number of cells in a simple microscopically. This technique is very similar to that used in blood cell counts. Scientists perform a direct microscopic count using a specially designed slide called a Petroff-Hausser cell counter/ counting chamber/ cytometer/ hemacytometer …

Food is any substance consumed to provide nutritional support for the body. Food contains essential nutrients such as carbohydrates, fats, proteins, vitamins or minerals. Food is ingested by an organism and assimilated by the organisms cells to provide energy, maintain life or stimulate growth. Classifications of food According to Martin …