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Classic Layout

Organization of Nuclear DNA in Eukaryotes

Each eukaryotic nucleus encloses a fixed number of chromosomes which contain the nuclear DNA. During most of a cell’s life, its chromosomes exist in a highly extended linear form. Prior to cell division, however, they condense into much more compact bodies which can be examined microscopically after staining. The duplication …

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Genome

Genome Structure in Viruses & Prokaryotes

The genomes of viruses and prokaryotes are very simple structures, although those of viruses show remarkable diversity (for a review see Dimmock et al. 2001). Most viruses have a single linear or circular genome but a few, such as reoviruses, bacteriophage φ6 and some plant viruses, have segmented RNA genomes. …

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DNA Reassociation Kinetics & Sequence Complexity

When double-stranded DNA in solution is heated, it denatures (‘melts’) releasing the complementary single strands. If the solution is cooled quickly the DNA remains in a single-stranded state. However, if the solution is cooled slowly reassociation/reannealing i. e. spontaneous realignment of two single DNA strands to re-form a DNA double …

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